The incidence of glossopharyngeal neuralgia (GPN) is low, but the pain of GPN is severe and seriously affects the patient's life. We report a case with GPN, who had pain relief after treatment with high-voltage pulsed radiofrequency (PRF) combined with duloxetine. This case indicates that high-voltage PRF combined with duloxetine may be an effective approach for reducing pain in patients with GPN, and duloxetine may have a potential advantage for patients with evoked pain.