Brucellosis, a significant zoonotic disease, poses a threat to both livestock and human health. Infections in livestock lead to abortion, infertility, and substantial economic losses in the industry. In humans, acute brucellosis can progress to a chronic condition, resulting in multisystemic infections with high morbidity and mortality rates. Additionally, the bioterrorism potential of certain Brucella species through aerosol transmission poses risks to laboratory workers and livestock handlers. Therefore, there is an urgent need for rapid and precise diagnosis of brucellosis in both animals and humans. Even with the availability of routine diagnostic techniques that are effective they frequently have some limitations. Biosensors, as innovative techniques, have demonstrated significant potential in detecting various pathogens with high efficiency. These biosensors can identify specific analytes, biomolecules of pathogenic bacteria, secreted antibodies against bacteria, and even the bacterial body in real time. Their high sensitivity, selectivity, and user-friendly configurations make them valuable tools for diagnostics. In this comprehensive review, beside the reviewing routine diagnostic tests for detecting brucellosis and discussing the positive and negative aspects of these methods, we explore different types of biosensors and their applications in diagnosing brucellosis. We hope to show how these advancements can result in quicker and more precise disease detection by offering a thorough evaluation of these technologies performance and contrasting it with more conventional diagnostic techniques. This improves patient outcomes by lowering the complications linked to delayed diagnosis in addition to advancing scientific knowledge of brucellosis.